Back to Blog
Cornus mas gold5/30/2023 ![]() ![]() It typically grows over time to 15-25' tall with a spread to 12-20' wide. Any queries (other than missing content) should be directed to the corresponding author for the article.Cornus mas, commonly known as cornelian cherry, is a deciduous shrub or small tree that is native to central and southern Europe into western Asia. ![]() Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Results depicted as bars indicate NO concentrations and are given as mean ± SD (n = 3). Representative results of one from at least three independent experiments are shown. Every experimental setting was performed in triplicate. Ag-NPs-CM and especially Au-NPs-CM efficiently inhibited NO production at both higher (1:10) and lower (1:100) concentrations. Nitric oxide (NO) release was measured as nitrite (NO-2) concentration in the supernatants of murine macrophages activated with 50 U/mL IFNγ and 10 ng/mL LPS alone (as a positive control for maximal NO production) or in presence of Au-NPs-CM or Ag-NPs-CM at 1:10 and 1:100 dilutions, respectively. Results depicted as bars representing LDH activity as a percentage of control untreated cells are given as mean ± SDįIGURE S2 Au-NPs-CM and Ag-NPs-CM significantly decrease NO release from pro-inflammatory stimulated murine macrophages. One representative of three independent experiments is shown. (b) Likewise, the MTT assay performed 48 hours after treatment did not show any significant differences in cell viability between the different culture settings, and thus no increased cellular death upon treatment with Ag-NPs-CM or Au-NPs-CM. (a) LDH concentrations measured in the supernatants of macrophage cultures 48 hours after treatment did not show any significant differences for LDH release between the different culture settings, and thus no cellular disruption or death due to the treatment with Ag-NPs-CM or Au-NPs-CM. Naïve and pro-inflammatory activated macrophage cultures were treated with silver and gold nanoparticles at 1:10 and 1:100 dilutions. 2012, 61, 1177.Įxd1370-SupInfo.docWord document, 63.5 KBįIGURE S1 Ag and Au NPs-CM do not affect macrophage viability in vitro. Chiorean, International Journal of Latest Research. Ag and Au NPs-CM represent a novel nanoparticle-based “green” technology which may provide an efficient tool for modern psoriasis therapy, circumventing immunosuppression-related side effects of biologicals. NPs-CM appear to repress NF-κB activation in macrophages, inhibiting the production of pro-inflammatory factors with causal role in psoriasis. Immunofluorescence confirmed that nanoparticles significantly reduced CD68-positive macrophages and their IL-12 and TNF-α production in human psoriasis plaques. Incubation of pro-inflammatory macrophages with nanoparticles significantly decreased the release of NO, IL-12 and TNF-α. The impact on psoriatic inflammation was assessed in vitro on pro-inflammatory macrophages, by clinical score, high-frequency ultrasonography and immunohistology of psoriasis plaques treated with Ag-NPs-CM, Au-NPs-CM or control. ![]() We aimed to understand how topically delivered silver and gold nanoparticles complexed with Cornus mas (Ag-NPs-CM, Au-NPs-CM) modulate inflammation in psoriasis at cellular and molecular level. New biomaterials based on nanoparticles (NPs) carrying polyphenols-rich extracts ( Cornus mas) recently showed promising anti-inflammatory activity in psoriasis. ![]()
0 Comments
Read More
Leave a Reply. |